Non-coding RNAs and translational regulatory process in Staphylococcus aureus: virulence and adaptation to environments
Led by F. Vandenesch (PU-PH) and K. Moreau (PU) with support of F. Couzon (engineer), in collaboration with P. Romby group (UPR 9002 CNRS, Strasbourg)
The team has pursued the task of characterizing S. aureus non-coding RNAs and their involvement in the regulation of virulence. Using original MAPS (MS2-Affinity Purification Coupled With RNA Sequencing) technology, we were able to identify target of several non-coding RNA (rsaA, rsaC, rsaG and rsaI) and demonstrate their role in regulation of virulence and adaptation to environment such as of oxidative stress response in context of nutritional deficiency. In parallel, we aim to determine the contribution of post transcriptional and translational regulatory processes to gene expression and virulence upon exposure to two major stress responses encountered during infection (oxidative and anti-toxinic antibiotic treatments).
These projects on regulatory RNAs and translation are conducted in close collaboration with P. Romby group (IBMC, Strasbourg) who brings the structural part of the various project whilst our group provide the functional (cellular, animal, translational...) contribution to the project. This collaboration led to 20 collaborative papers in 19 years period and was supported by several ANR grants.
RsaC non-coding RNA structure and fonction. From Lalaouna et al., 2019.
Recent publications:
Lalaouna D, Baude J, Wu Z, Tomasini A, Chicher J, Marzi S, Vandenesch F, Romby P, Caldelari I, Moreau K. RsaC sRNA modulates the oxidative stress response of Staphylococcus aureus during manganese starvation. Nucleic Acids Res. 2019 Oct 10;47(18):9871-9887. doi: 10.1093/nar/gkz728. PubMed PMID: 31504767
Desgranges E, Marzi S, Moreau K, Romby P, Caldelari I. Noncoding RNA. Microbiol Spectr. 2019 Mar;7(2). doi: 10.1128/microbiolspec.GPP3-0038-2018. PubMed PMID: 31004423.
Bronesky D, Desgranges E, Corvaglia A, François P, Caballero CJ, Prado L,Toledo-Arana A, Lasa I, Moreau K, Vandenesch F, Marzi S, Romby P, Caldelari I. A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus. EMBO J. 2019 Mar 15;38(6). pii: e99363. doi:10.15252/embj.201899363. Epub 2019 Feb 13. PubMed PMID: 30760492
Tomasini A, Moreau K, Chicher J, Geissmann T, Vandenesch F, Romby P, Marzi S, Caldelari I. The RNA targetome of Staphylococcus aureus non-coding RNA RsaA: impact on cell surface properties and defense mechanisms. Nucleic Acids Res. 2017 Jun 20;45(11):6746-6760. doi: 10.1093/nar/gkx219. PubMed PMID: 28379505
Bronesky D, Wu Z, Marzi S, Walter P, Geissmann T, Moreau K, Vandenesch F, Caldelari I, Romby P. Staphylococcus aureus RNAIII and Its Regulon Link Quorum Sensing, Stress Responses, Metabolic Adaptation, and Regulation of Virulence Gene Expression. Annu Rev Microbiol. 2016 Sep 8;70:299-316. doi:10.1146/annurev-micro-102215-095708. Epub 2016 Jul 6. Review. PubMed PMID: 27482744.
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