CIRI Seminar: Dr. Petr CHLANDA
“Zooming in on replication cycle of respiratory viruses by in situ cryo-electron tomography”
| When |
May 18, 2021
from 11:00 AM to 12:00 PM |
|---|---|
| Where | visioconférence |
| Contact Name | Marlène Dreux |
Dr Petr CHLANDA (Heidelberg university): « Zooming in on replication cycle of respiratory viruses by in situ cryo-electron tomography »
Abstract: We have established an in situ cryo-correlative light and electron tomography workflow to study cellular organelles are their interactions with viruses during infection in unperturbed conditions and at a molecular resolution. This method allowed us to describe the architecture of lamellar bodies present in type II pneumocytes and to capture influenza A virus entry intermediates. In addition, our data revealed a viral RNA network inside double-membrane vesicles and assembly steps of SARS-CoV-2.