Mechanisms of Staphylococcus capitis NRCS-A pathogenesis in neonates

Led by M. Butin (MCU-PH) and F.Laurent (PU-PH), with assistance P. Martin Simoes (engineer) and M. Bergot (assistant-engineer), in collaboration with T. Wirth (PU, EPHE, Museum d’Histoire Naturelle, Paris).

Staphylococcus capitis NRCS-A has recently emerged as a major multidrug-resistant pathogen involved in nosocomial sepsis in neonates worldwide (cf chapter  achievement). We aim to explore the specific affinity of NRCS-A for the neonates on the basis of genomic and transcriptomic approaches. Using in vitro (Caco-2 and M-cell-like model), ex vivo (mucosal explant from neonates) and animal models, we aim to better understand the pathophysiology of gut colonization, translocation and infection with NRCS-A. In addition, new strategies to eradicate NRCS-A from NICU settings will be defined: (i) by exploring the mechanism of persistence of NRCS-A on inert surfaces (biofilm production, resistance to antiseptic molecules) and (ii) by testing alternative disinfection procedures (including steam cleaner). Finally, the exploitation of the clinical data of a prospective nationwide cohort (Epipage2) will allow for the evaluation of the long-term impact of neonatal gut colonization/infection with NRCS-A.