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Vous êtes ici : Accueil / Équipes / Marvel J - ICL / Actualités / Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions

Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions

S.Wang, M.Prieux, S.de Bernard, M.Dubois, D.Laubreton , S.Djebali, M.Zala, C.Arpin, L.Genestier, Y.Leverrier, O.Gandrillon, F.Crauste, W.Jiang, J.Marvel

Abstract

To investigate the impact of paracrine IL-2 signals on memory precursor (MP) cell differentiation, we activated CD8 T cell in vitro in the presence or absence of exogenous IL-2 (ex-IL-2). We assessed memory differentiation by transferring these cells into virus-infected mice. Both conditions generated CD8 T cells that participate in the ongoing response and gave rise to similar memory cells. Nevertheless, when transferred into a naive host, T cells activated with ex-IL-2 generated a higher frequency of memory cells displaying increased functional memory traits. Single-cell RNA-seq analysis indicated that without ex-IL-2, cells rapidly acquire an MP signature, while in its presence they adopted an effector signature. This was confirmed at the protein level and in a functional assay. Overall, ex-IL-2 delays the transition into MP cells, allowing the acquisition of effector functions that become imprinted in their progeny. These findings may help to optimize the generation of therapeutic T cells.

Keywords: Biological sciences; Immune system evolution; Immunology; Molecular biology.

PMID: 38510150 ; PMCID: PMC10952031  ; DOI: 10.1016/j.isci.2024.109411

https://www.cell.com/iscience/fulltext/S2589-0042(24)00632-1

 

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