Multicenter study on nosocomial transmission of SARS-CoV-2 virus - immunological study (NOSO-COR IMMUNO)
There are still many uncertainties about the existence of protective immunity and the duration of the presence of antibodies in patients infected with SARS-CoV-2. Serological tests are appropriate tools for epidemiological investigations to assess the persistence of antibodies over time.
The main objective of this ancillary study is to describe the serological response in people infected with SARS-CoV-2 over time after the resolution of initial infectious episode.
Secondary objectives
1. Characterization of T CD8 populations specific to virus, phenotype and functional capacity, present in the circulation - peripheral blood lymphocytes
2. Titration of neutralizing antibodies vs. facilitators
3. Comparison of the characteristics of immune response memory in patients
4. Study of immune restoration post-COVID-19
5. Building a collection of saliva and blood samples at HCL
The results would help to assess seroprevalence in patients with confirmed SARS-CoV-2 infection.
They would make it possible to know the characteristics of the patients and changes in their post-infection serological profile
Characteristics of the immune response memory in patients: humoral memory, circulating antibodies (spike protein specificity of the virus, and neutralizing or facilitating functionality), mucosal polyreactive IgA, and cellular memory, T-cell repertoire, cytotoxic CD8 T lymphocytes (phenotype and function) and NK memory (receptor repertoire, exhaustion markers and functionality) would also be compared
A correlation between the parameters of the memory response and the severity of the primary infection will be sought.
These comparisons may be useful to:
1) Define the frequency of patients developing humoral and /or cellular immunity
2) Possibly anticipate the profile of non-responders to exposure to the virus
3) Identify the correlates of protection that should be sought to be induced with the vaccines under development