NLRP3 and inflammasomes

• Optogenetically controlled inflammasome activation demonstrates two phases of cell swelling during pyroptosis.

  Nadjar J, Monnier S, Bastien E, Huber AL, Oddou C, Bardoulet L, Leloup HB, Ichim G, Vanbelle C, Py BF, Destaing O, Petrilli V. 

       Sci Signal. 2024 Apr 23;17(833):eabn8003. doi: 10.1126/scisignal.abn8003.

• Functional analysis of NLRP3 variants provides insight into inflammasome regulation.

  Py BF. 

       Nat Immunol. 2025 Mar;26(3):337-339. doi: 10.1038/s41590-025-02093-y.

• [Functional diversity of NLRP3 gain-of-function mutations and impact on the diagnosis of CAPS hereditary auto-inflammation disease].

  Cosson C, Riou R, Py BF. 

       Med Sci (Paris). 2024 Oct;40(10):707-710. doi: 10.1051/medsci/2024110. French

• Implication of the LRR Domain in the Regulation and Activation of the NLRP3 Inflammasome.

       Cescato M, Zhu YYJ, Le Corre L, Py BF, Georgin-Lavialle S, Rodero MP. 

       Cells. 2024 Aug 16;13(16):1365. doi: 10.3390/cells13161365. Review.

• Functional diversity of NLRP3 gain-of-function mutants associated with CAPS autoinflammation
  C. Cosson, R. Riou, D. Patoli, T. Niu, A. Rey, M. Groslambert, C. De Rosny, E. Chatre, O. Allatif, T. Henry, F. Venet, F. Milhavet, G. Boursier, A. Belot, Y. Jamilloux, E. Merlin, A. Duquesne, G. Grateau, L. Savey, A. T. J. Maria, A. Pagnier, S. Poutrel, O. Lambotte, C. Mallebranche, S. Ardois, O. Richer, I. Lemelle, F. Rieux-Laucat, B. Bader-Meunier, Z. Amoura, I. Melki, L. Cuisset, I. Touitou, M. Geyer, S. Georgin-Lavialle, and B. F. Py

       J. Exp. Med. 2024 May 6;221(5):e20231200; doi: 10.1084/jem.20231200.

Video summary by Benedicte Py:

• Monitoring NLRP3 Inflammasome Activation and Exhaustion in Clinical Samples: A Refined Flow Cytometry Protocol for ASC Speck Formation Measurement Directly in Whole Blood after Ex Vivo Stimulation

Rémy Coudereau, Morgane Gossez, Bénédicte F Py, Thomas Henry, Anne-Claire Lukaszewicz, Guillaume Monneret and Fabienne Venet

Cells 2022, 11(20), 3306; doi:10.3390/cells11203306

• Role of inflammasome NLRP3 in the pathophysiology of viral infections: A focus on SARS-CoV-2 infection

Marlène Chemarin, Océane Dufies, Anastassia Mazet , Eva Mellan, Rémy Coudereau, Bénédicte F Py, Laurent Boyer, Fabienne Venet

Med Sci (Paris). 2022 Jun-Jul;38(6-7):545-552. doi: 10.1051/medsci/2022080. Review. [French].

• Evidence for Constitutive Microbiota-Dependent Short-Term Control of Food Intake in Mice: Is There a Link with Inflammation, Oxidative Stress, Endotoxemia, and GLP-1?

• NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly

Tingting Niu, Charlotte De Rosny, Séverine Chautard, Amaury Rey, Danish Patoli,
Marine Groslambert, Camille Cosson, Brice Lagrange, Zhirong Zhang, Orane Visvikis, Sabine Hacot, Maggy Hologne, Olivier Walker, Jeimin Wong, Ping Wang, Roméo Ricci, Thomas Henry, Laurent Boyer, Virginie Petrilli & Bénédicte F. Py

Nat Commun. 2021 Oct 6;12(1):5862. doi: 10.1038/s41467-021-26142-w

• Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice.

• Spotlight on the NLRP3 inflammasome pathway.

Groslambert M, Py BF.
J Inflamm Res. 2018 Sep 25;11:359-374. doi: 10.2147/JIR.S141220. Review.

• Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11.

Lagrange B, Benaoudia S, Wallet P, Magnotti F, Provost A, Michal F, Martin A, Di Lorenzo F, Py BF, Molinaro A, Henry T.
Nat Commun. 2018 Jan 16;9(1):242. doi: 10.1038/s41467-017-02682-y

• [NLRP3, un inflammasome sous contrôle.[Regulation of the NLRP3 inflammasome.]

Groslambert M, Py BF.
Med Sci (Paris). 2018 Jan;34(1):47-53. doi: 10.1051/medsci/20183401013. Review. [French]

• Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing.

Teixeira M, Py BF, Bosc C, Laubreton D, Moutin MJ, Marvel J, Flamant F, Markossian S.
Sci Rep. 2018 Jan 11;8(1):474. doi: 10.1038/s41598-017-18826-5

• IFN-gamma extends the immune functions of Guanylate Binding Proteins to inflammasome independent antibacterial activities during Francisella novicida infection.

Wallet P, Benouadia S, Mosnier A, Lagrange B, Martin A, Lindgren H, Golovlev I, Michal F, Basso P, Djebali S, Provost A, Allatif O, Meunier E, Broz P, Yamamoto M, Py BF, Faudry E, Sjostedt A, Henry T.
Plos Pathogens. 2017 Oct 2. doi: 10.1371/journal.ppat.1006630

• Familial Mediterranean fever mutations are hypermorphic mutations that specifically decrease the activation threshold of the Pyrin inflammasome.

Jamilloux Y, Lefeuvre L, Magnotti F, Martin A, Benezech S, Allatif O, Penel-Page M, Hentgen V, Sève P, Gerfaud-Valentin M, Duquesne A, Desjonquères M, Laurent A, Rémy-Piccolo V, Cimaz R, Cantarini L, Bourdonnay E, Walzer T, Py BF, Belot A, Henry T.
Rheumatology. 2017 Oct 12. doi: 10.1093/rheumatology/kex373

• Roles of Caspases in Necrotic Cell Death.

Yuan J, Najafov A, Py BF.
Cell. 2016 Dec 15;167(7):1693-1704. doi: 10.1016/j.cell.2016.11.047. Review.

• [Interleukin-1, inflammasome and autoinflammatory diseases.]

Jamilloux Y, Bourdonnay E, Gerfaud-Valentin M, Py BF, Lefeuvre L, Barba T, Broussolle C, Henry T, Seve P.
Rev Med Interne. 2016. S0248-8663(16):30472-6. doi: 10.1016/j.revmed.2016.07.007. Review. [French]

• Design, synthesis, and evaluation of acrylamide derivatives as direct NLRP3 inflammasome inhibitors.

Cocco M, Miglio G, Giorgis M, Garella D, Marini E, Costale A, Regazzoni L, Vistoli G, Orioli M, Massulaha-Ahmed R, Detraz-Durieux I, Groslambert M, Py BF, Bertinaria M.

Chem Med Chem. 2016. 11(16):1790-803. doi: 10.1002/cmdc.201600055

• Caspase-11 controls interleukin-1b release through degradation of TRPC1.

Py BF, Jin M, Desai BN, Penumaka A, Zhu H, Kober M, Dietrich A, Lipinski MM, Henry T, Clapham DE, Yuan J.
Cell Reports. 2014. 97(2):292-6. doi: 10.1016/j.celrep.2014.02.015

• Caspase-1 activity affects AIM2 speck formation/stability through a negative feedback loop.

Juruj C, Lelogeais V, Pierini R, Perret M, Py BF, Jamilloux Y, Broz P, Faure M, Henry T.
Front Cell Infect Microbiol. 2013. 3-14. doi: 10.3389/fcimb.2013.00014

• Deubiquitination of NLRP3 by BRCC3 critically regulates inflammasome activity.

Py BF, Kim MS, Vakifahmetoglu-Norberg H, Yuan J.
Mol. Cell. 2013. 49(2):331-8. doi: 10.1016/j.molcel.2012.11.009

Réponse immunitaire au sepsis

• PD-L1⁺ plasma cells suppress T lymphocyte responses in patients with sepsis and mouse sepsis models.

  Gossez M, Vigneron C, Vandermoeten A, Lepage M, Courcol L, Coudereau R, Paidassai H, Jallades L, Lopez J, Kandara K, Ortillon M, Mommert M, Fabri A, Peronnet E, Grosjean C, Buisson M, Lukaszewicz AC, Rimmelé T, Argaud L, Cour M; REALISM study group; RICO study group; Py BF, Thaunat O, Defrance T, Monneret G, Venet F. 

       Nat Commun. 2025 Mar 28;16(1):3030. doi: 10.1038/s41467-025-57706-9. Open Access

• Shadows and lights in sepsis immunotherapy.

  Monneret G, Haem Rahimi M, Lukaszewicz AC, Venet F, Gossez M. 

       Expert Opin Pharmacother. 2024 Nov;25(16):2125-2133. doi: 10.1080/14656566.2024.2418987.  Review

• T cell response against SARS-CoV-2 persists after one year in patients surviving severe COVID-19.

Venet F, Gossez M, Bidar F, Bodinier M, Coudereau R, Lukaszewicz AC, Tardiveau C, Brengel-Pesce K, Cheynet V, Cazalis MA, Pescarmona R, Garnier L, Ortillon M, Buisson M, Bouscambert-Duchamp M, Morfin-Sherpa F, Casalegno JS, Conti F, Rimmelé T, Argaud L, Cour M, Saadatian-Elahi M, Henaff L, Vanhems P, Monneret G. 

eBioMedicine. 2022 Apr;78:103967. doi: 10.1016/j.ebiom.2022.103967

• Immune response to three doses of mRNA SARS-CoV-2 vaccines in CD19-targeted chimeric antigen receptor T cell immunotherapy recipients.

Sesques P, Bachy E, Ferrant E, Safar V, Gossez M, Morfin-Sherpa F, Venet F, Ader F. 

Cancer Cell. 2022 Jan 25:S1535-6108(22)00012-5. doi: 10.1016/j.ccell.2022.01.010

• Longitudinal assessment of IFN-I activity and immune profile in critically ill COVID-19 patients with acute respiratory distress syndrome.

Venet F, Cour M, Rimmelé T, Viel S, Yonis H, Coudereau R, Amaz C, Abraham P, Monard C, Casalegno JS, Brengel-Pesce K, Lukaszewicz AC, Argaud L, Monneret G; RICO study group. 

Crit Care. 2021 Apr 12;25(1):140. doi: 10.1186/s13054-021-03558-w

• COVID-19: What type of cytokine storm are we dealing with?

Monneret G, Benlyamani I, Gossez M, Bermejo-Martin JF, Martín-Fernandez M, Sesques P, Wallet F, Venet F. 

J Med Virol. 2021 Jan;93(1):197-198. doi: 10.1002/jmv.26317

Cochlin

• Cochlin produced by follicular dendritic cells promotes anti-bacterial innate immunity.

Py BF, Gonzalez SF, Long K, Kim MS, Kim YA, Zhu H, Yao J, Degauque N, Villet R, Ymele-leki P, Gadjeva M, Pier GB, Carroll MC, Yuan J
Immunity. 2013. 38(5):1063-72. doi: 10.1016/j.immuni.2013.01.015

• Role of protein misfolding in DFNA9 hearing loss.

Yao J, Py BF, Zhu H, Bao J, Yuan J.
Journal of Biological Chemistry. 2010. 285(20):14909-19. doi: 10.1074/jbc.M110.106724

Other topics

• Genome-wide analysis reveals mechanisms modulating autophagy in normal brain aging and in Alzheimer’s disease.

Lipinski MM, Zheng B, Lu T, Yan Z, Py BF, Ng A, Xavier RJ, Li C, Yankner BA, Scherzer CR, Yuan J.
Proc Natl Acad Sci USA. 2010. 107(32):14164-9. doi: 10.1073/pnas.1009485107

• A genome-wide siRNA screen reveals multiple mTORC1 independent signaling pathways regulating autophagy under normal nutritional conditions.

Lipinski MM, Hoffman G, Ng A, Zhou W, Py BF, Hsu E, Liu X, Eisenberg J, Liu J, Blenis J, Xavier RJ, Yuan J.
Developmental Cell. 2010. 18(6):1041-1052. doi: 10.1016/j.devcel.2010.05.005

• The phospholipid scramblases 1 and 4 are cellular receptors for the secretory leucocyte protease inhibitor and interact with CD4 at the plasma membrane.

Py B, Basmaciogullari S, Bouchet J, Zarka M, Moura IC, Benhamou M, Monteiro RC, Hocini H, Madrid R, Benichou S.
PLoS ONE. 2009. 4(3).  doi: 10.1371/journal.pone.0005006

• A critical role of eEF-2K in mediating autophagy in response to multiple cellular stresses.

Py BF, Boyce M, Yuan J.
Autophagy. 2009. 5(3):393-6. doi: 10.4161/auto.5.3.7762

• Fission and selective fusion govern mitochondrial segregation and elimination by autophagy.

Twig G, Elorza A, Molina A, Mohamed H, Wikstrom JD, Stiles L, Walzer G, Haigh SE, Katz S, Las G, Alroy J, Wu M, Py BF, Yuan J, Deeney JT, Corkey BE, Shirihai OS.
EMBO Journal. 2008. 27(2):433-46. doi: 10.1038/sj.emboj.7601963

• A pharmaco-proteomic approach implicates eukaryotic elongation factor 2 kinase in ER-stress-induced cell death.

Boyce M, Py BF, Ryazanov AG, Minden JS, Long K, Ma D, Yuan J.
Cell Death and Differentiation. 2008. doi: 10.1038/sj.cdd.4402296

• The Siva protein is a novel intracellular ligand of the CD4 receptor that promotes HIV-1 envelope-induced apoptosis in T-lymphoid cells.

Py B, Bouchet J, Jacquot G, Sol-Foulon N, Basmaciogullari S, Schwartz O, Biard-Piechaczyk M, Benichou S.
Apoptosis. 2007. 12(10):1879-92. doi: 10.1007/s10495-007-0106-4

• Autophagy limits Listeria monocytogenes intracellular growth in the early phase of primary infection.

Py BF, Lipinski MM, Yuan J.
Autophagy. 2007. 3(2):117-25. doi: 10.4161/auto.3618

• Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway.

Py B, Slomianny C, Auberger P, Petit PX, Benichou S.
Journal of Immunology. 2004. 172(7):4008-4017. doi: 10.4049/jimmunol.172.7.4008

Book chapters

• Endoplasmic reticulum stress response in cell death and cell survival.

Boyce M, Lipinski MM, Py BF, Yuan J.
in « Apoptosis: Physiology and Pathology of Cell Death » edited by Reed JC and Green DR, New York: Cambridge University Press; August 2011.