Séminaire CIRI (05.11.2024): Lea CHAZOT-FRANGUIADAKIS

 Lea CHAZOT-FRANGUIADAKIS, Laboratoire de physique, Équipe Matière & complexité, ENS-Lyon, “Characterization of Viral Particles by Nanopores ”

Abstract: Our research focuses on the development of a nanopore-based device for real-time detection and characterisation of viruses at the single particle level. This platform offers two modes: targeted detection and quantification of a specific virus (candidate approach) or unbiased identification of viral particles (unbiased approach) in complex samples. The technique is based on the optical detection of fluorescently labelled viruses transported through nanopores. In this presentation, I will show that thanks to the fluorescent labelling, the degree of specificity of this method can be modulated according to the application  to target, for example, the whole enveloped particle, the viral genome or a surface protein of interest. I will then show that, unlike traditional methods such as PCR or antigenic assays, our system detects viral particles in their entirety, giving access to empty versus full particle ratios. This versatile approach enables precise and rapid quantification of particle concentration (around 30 min). We have applied this method to numerous viral types (AAV, HBV, HIV, SC2, InfV, ...) and we are keen to develop new collaborations on new viral types and/or new applications.

  Host : Philippe Mangeot